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invitro biotin ligase kit avidity  (New England Biolabs)


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    New England Biolabs invitro biotin ligase kit avidity
    Invitro Biotin Ligase Kit Avidity, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/invitro+biotin+ligase+kit+avidity/Endoproteinase+LysC/pm39909035-652-13-22
    Average 96 stars, based on 112 article reviews
    invitro biotin ligase kit avidity - by Bioz Stars, 2026-10
    96/100 stars

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    Article Title: Structural basis for complement receptor engagement and virus neutralization through Epstein-Barr virus gp350.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 goat anti-mouse IgG (H+L chain) ThermoFisher Scientific Cat# A11001; RRID: AB_2534069 Anti-human CD27 QD605 ThermoFisher Scientific Cat# Q10065; RRID: AB_2556450 Anti-human CD14 QD800 ThermoFisher Scientific Cat# Q10064; RRID: AB_2556449 IgG BV421 BD Biosciences Cat# 562581; RRID: AB_2737665 IgM PerCP/Cy5.5 BD Biosciences Cat# 561285; RRID: AB_10611998 Anti-human CD20 APC-Cy7 BD Biosciences Cat# 335794; RRID: AB_399972 Anti-human IgD FITC Southern Biotech Cat# 2030-02; RRID: AB_2795624 Anti-human CD19 ECD Beckman Coulter Cat# A07770; RRID: AB_2940901 Anti-human CD14 BV510 BioLegend Cat# 301842; RRID: AB_2561946 Anti-human CD56 BV510 BioLegend Cat# 318340; RRID: AB_2561944 Anti-human CD3 BV510 BioLegend Cat# 317332; RRID: AB_2561943 Anti-human CD21 PE/Cy5 BD Biosciences Cat# 551064; RRID: AB_394028 Anti-human CD21 clone 1048 BD Biosciences Cat# 552727; RRID: AB_394446 Anti-human CD27 BV605 BioLegend Cat# 302830; RRID: AB_2561450 Anti-CD38 AF680 Vaccine Research Center, NIAID, NIH N/A Anti-HA (C179) Takara Bio Cat# M145 Cy137B03 This paper N/A Cy137C02 This paper N/A Cy137D07 This paper N/A Cy137D09 This paper N/A Cy137E04 This paper N/A Cy651B04 This paper N/A Cy651D06 This paper N/A Cy651F11 This paper N/A Cy651G12 This paper N/A Cy651H02 This paper N/A 769A9 This paper N/A 770E11 This paper N/A Bacterial and virus strains AKATA EBV GFP Molesworth et al.56 N/A B95-8/F Delecluse et al.57 N/A Biological samples Serum and PBMC from healthy blood bank donors National Institutes of Health Warren G. Magnuson Clinical Center N/A Chemicals, peptides, and recombinant proteins EBV gp350-Avi-His This paper N/A Kifunensine Sigma-Aldrich Cat# K1140 Streptavidin PE ThermoFisher Scientific Cat# S21388 Streptavidin APC ThermoFisher Scientific Cat# S32362 PE-Cy5 streptavidin BD Biosciences Cat# 554062; RRID: AB_10053563 (Continued on next page) Immunity 58, 295–308.e1–e5, February 11, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays PreScission Protease Cytiva Cat# 27084301 InVitro Biotin Ligase Kit Avidity Cat# BirA-500 Endoproteinase LysC New England Biolabs Cat# P8109S Endoglycosidase H New England Biolabs Cat# P0702L LIVE/DEAD Fixable Aqua Dead Cell Stain Kit ThermoFisher Scientific Cat# L34957 Superscript III Reverse Transcriptase ThermoFisher Scientific Cat# 18080093 Expi293 Expression System Kit ThermoFisher Scientific Cat# A14635 Deposited data Crystal structure of EBV gp350 with human CR2 (CD21) This paper PDB code: 8SM0 Crystal structure of EBV gp350 with macaque Cy137C02 Fab This paper PDB code: 8SIC Crystal structure of EBV gp350 with macaque Cy651H02 Fab This paper PDB code: 8SGN Crystal structure of EBV gp350 with human 769A9 Fab This paper PDB code: 8SM1 Crystal structure of macaque Cy137C02 Fab This paper PDB code: 8SEF Crystal structure of macaque Cy137D09 Fab This paper PDB code: 8SGG Crystal structure of human 770E11 Fab This paper PDB code: 8SGA EM EBV gp350 with human 769A9 Fab This paper N/A EM EBV gp350 with human 770E11 Fab This paper N/A Cy137B03 This paper GenBank: PQ539289/PQ539290 Cy137C02 This paper GenBank: PQ539291/PQ539292 Cy137D07 This paper GenBank: PQ539293/PQ539294 Cy137D09 This paper GenBank: PQ539295/PQ539296 Cy137E04 This paper GenBank: PQ539297/PQ539298 Cy651B04 This paper GenBank: PQ539299/PQ539300 Cy651D06 This paper GenBank: PQ539301/PQ539302 Cy651F11 This paper GenBank: PQ539303/PQ539304 Cy651G12 This paper GenBank: PQ539305/PQ539306 Cy651H02 This paper GenBank: PQ539307/PQ539308 769A9 This paper GenBank: PQ539149/PQ539150 770E11 This paper GenBank: PQ539151/PQ539152 Experimental models: Cell lines Expi293F ThermoFisher Scientific Cat# A14527 Mouse hybridoma 72A1 ATCC HB-168 Akata BX1 Molesworth et al.56 N/A AGS ATCC Cat# CRL-1739; RRID: CVCL_0139 SVKCR2 Li et al.58 RRID: CVCL_YD67 Experimental models: Organisms/strains Cynomolgus macaques Vaccine Research Center, NIAID, NIH N/A Oligonucleotides EBV gp350 Q122N This paper N/A EBV gp350 D163N/164bS This paper N/A EBV gp350 162-164AAA This paper N/A EBV gp350 D296R This paper N/A (Continued on next page) e2 Immunity 58, 295–308.e1–e5, February 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER EBV gp350 D296R/P158R This paper N/A EBV gp350 D296R/I160R This paper N/A EBV gp350 D296R/K161A This paper N/A EBV gp350 D296R/K161D This paper N/A Recombinant DNA pCMV/R-gp350 D1-3 Kanekiyo et al.34 N/A pVRC/R-gp350 D1-3-AviHis This paper N/A pCMV/R-CR21-Fc This paper N/A Software and algorithms Flowjo Flowjo http://www.flowjo.com Prism Graphpad http://www.graphpad.com Octet Data analysis software 9.0 FortéBio/Sartorius https://www.sartorius.com Biorender Biorender http://www.biorender.com PyMOL Schrödinger, Inc. https://pymol.org/2/ Phenix.refine Phenix software suite https://phenix-online.org/ Coot MRC Laboratory of Molecular Biology in Cambridge https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ UCSF ChimeraX UCSF https://www.cgl.ucsf.edu/chimerax Other Captia EBV VCA (P-18) IgG Kit Trinity Biotech Cat# 2324700 Ni Sepharose Cytiva Cat# 17531802 rmp Protein A Sepharose Fast Flow resin Cytiva Cat# 17513803 Tecnai T20 FEI Company N/A Streptavidin biosensors Sartorius Cat# 18-5019 Octet HTX instrument FortéBio/Sartorius N/A

    Reverse Transcription:

    Article Title: Structural basis for complement receptor engagement and virus neutralization through Epstein-Barr virus gp350.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 goat anti-mouse IgG (H+L chain) ThermoFisher Scientific Cat# A11001; RRID: AB_2534069 Anti-human CD27 QD605 ThermoFisher Scientific Cat# Q10065; RRID: AB_2556450 Anti-human CD14 QD800 ThermoFisher Scientific Cat# Q10064; RRID: AB_2556449 IgG BV421 BD Biosciences Cat# 562581; RRID: AB_2737665 IgM PerCP/Cy5.5 BD Biosciences Cat# 561285; RRID: AB_10611998 Anti-human CD20 APC-Cy7 BD Biosciences Cat# 335794; RRID: AB_399972 Anti-human IgD FITC Southern Biotech Cat# 2030-02; RRID: AB_2795624 Anti-human CD19 ECD Beckman Coulter Cat# A07770; RRID: AB_2940901 Anti-human CD14 BV510 BioLegend Cat# 301842; RRID: AB_2561946 Anti-human CD56 BV510 BioLegend Cat# 318340; RRID: AB_2561944 Anti-human CD3 BV510 BioLegend Cat# 317332; RRID: AB_2561943 Anti-human CD21 PE/Cy5 BD Biosciences Cat# 551064; RRID: AB_394028 Anti-human CD21 clone 1048 BD Biosciences Cat# 552727; RRID: AB_394446 Anti-human CD27 BV605 BioLegend Cat# 302830; RRID: AB_2561450 Anti-CD38 AF680 Vaccine Research Center, NIAID, NIH N/A Anti-HA (C179) Takara Bio Cat# M145 Cy137B03 This paper N/A Cy137C02 This paper N/A Cy137D07 This paper N/A Cy137D09 This paper N/A Cy137E04 This paper N/A Cy651B04 This paper N/A Cy651D06 This paper N/A Cy651F11 This paper N/A Cy651G12 This paper N/A Cy651H02 This paper N/A 769A9 This paper N/A 770E11 This paper N/A Bacterial and virus strains AKATA EBV GFP Molesworth et al.56 N/A B95-8/F Delecluse et al.57 N/A Biological samples Serum and PBMC from healthy blood bank donors National Institutes of Health Warren G. Magnuson Clinical Center N/A Chemicals, peptides, and recombinant proteins EBV gp350-Avi-His This paper N/A Kifunensine Sigma-Aldrich Cat# K1140 Streptavidin PE ThermoFisher Scientific Cat# S21388 Streptavidin APC ThermoFisher Scientific Cat# S32362 PE-Cy5 streptavidin BD Biosciences Cat# 554062; RRID: AB_10053563 (Continued on next page) Immunity 58, 295–308.e1–e5, February 11, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays PreScission Protease Cytiva Cat# 27084301 InVitro Biotin Ligase Kit Avidity Cat# BirA-500 Endoproteinase LysC New England Biolabs Cat# P8109S Endoglycosidase H New England Biolabs Cat# P0702L LIVE/DEAD Fixable Aqua Dead Cell Stain Kit ThermoFisher Scientific Cat# L34957 Superscript III Reverse Transcriptase ThermoFisher Scientific Cat# 18080093 Expi293 Expression System Kit ThermoFisher Scientific Cat# A14635 Deposited data Crystal structure of EBV gp350 with human CR2 (CD21) This paper PDB code: 8SM0 Crystal structure of EBV gp350 with macaque Cy137C02 Fab This paper PDB code: 8SIC Crystal structure of EBV gp350 with macaque Cy651H02 Fab This paper PDB code: 8SGN Crystal structure of EBV gp350 with human 769A9 Fab This paper PDB code: 8SM1 Crystal structure of macaque Cy137C02 Fab This paper PDB code: 8SEF Crystal structure of macaque Cy137D09 Fab This paper PDB code: 8SGG Crystal structure of human 770E11 Fab This paper PDB code: 8SGA EM EBV gp350 with human 769A9 Fab This paper N/A EM EBV gp350 with human 770E11 Fab This paper N/A Cy137B03 This paper GenBank: PQ539289/PQ539290 Cy137C02 This paper GenBank: PQ539291/PQ539292 Cy137D07 This paper GenBank: PQ539293/PQ539294 Cy137D09 This paper GenBank: PQ539295/PQ539296 Cy137E04 This paper GenBank: PQ539297/PQ539298 Cy651B04 This paper GenBank: PQ539299/PQ539300 Cy651D06 This paper GenBank: PQ539301/PQ539302 Cy651F11 This paper GenBank: PQ539303/PQ539304 Cy651G12 This paper GenBank: PQ539305/PQ539306 Cy651H02 This paper GenBank: PQ539307/PQ539308 769A9 This paper GenBank: PQ539149/PQ539150 770E11 This paper GenBank: PQ539151/PQ539152 Experimental models: Cell lines Expi293F ThermoFisher Scientific Cat# A14527 Mouse hybridoma 72A1 ATCC HB-168 Akata BX1 Molesworth et al.56 N/A AGS ATCC Cat# CRL-1739; RRID: CVCL_0139 SVKCR2 Li et al.58 RRID: CVCL_YD67 Experimental models: Organisms/strains Cynomolgus macaques Vaccine Research Center, NIAID, NIH N/A Oligonucleotides EBV gp350 Q122N This paper N/A EBV gp350 D163N/164bS This paper N/A EBV gp350 162-164AAA This paper N/A EBV gp350 D296R This paper N/A (Continued on next page) e2 Immunity 58, 295–308.e1–e5, February 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER EBV gp350 D296R/P158R This paper N/A EBV gp350 D296R/I160R This paper N/A EBV gp350 D296R/K161A This paper N/A EBV gp350 D296R/K161D This paper N/A Recombinant DNA pCMV/R-gp350 D1-3 Kanekiyo et al.34 N/A pVRC/R-gp350 D1-3-AviHis This paper N/A pCMV/R-CR21-Fc This paper N/A Software and algorithms Flowjo Flowjo http://www.flowjo.com Prism Graphpad http://www.graphpad.com Octet Data analysis software 9.0 FortéBio/Sartorius https://www.sartorius.com Biorender Biorender http://www.biorender.com PyMOL Schrödinger, Inc. https://pymol.org/2/ Phenix.refine Phenix software suite https://phenix-online.org/ Coot MRC Laboratory of Molecular Biology in Cambridge https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ UCSF ChimeraX UCSF https://www.cgl.ucsf.edu/chimerax Other Captia EBV VCA (P-18) IgG Kit Trinity Biotech Cat# 2324700 Ni Sepharose Cytiva Cat# 17531802 rmp Protein A Sepharose Fast Flow resin Cytiva Cat# 17513803 Tecnai T20 FEI Company N/A Streptavidin biosensors Sartorius Cat# 18-5019 Octet HTX instrument FortéBio/Sartorius N/A



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    invitro biotin ligase kit avidity  (New England Biolabs)
    96
    New England Biolabs invitro biotin ligase kit avidity
    Invitro Biotin Ligase Kit Avidity, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/invitro+biotin+ligase+kit+avidity/Endoproteinase+LysC/pm39909035-652-13-22
    Average 96 stars, based on 1 article reviews
    invitro biotin ligase kit avidity - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

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